AllTest Adenovirus Pneumoniae Antigen Lateral Flow Immunochromatographic Assays Rapid Test Cassette Swab

AllTest Adenovirus Antigen Lateral Flow Immunochromatographic Assays Rapid Test Cassette Swab

Applications:

The Adenovirus Antigen Rapid Test Cassette (Swab) is a rapid chromatographic immunoassay for the qualitative detection of Adenovirus antigen in eye conjunctive swab, throat swab and nasal swab as an aid in the diagnosis of adenovirus infections.

Description:

Although there are a variety of viruses that can cause infections in the lower respiratory tract in children and adults, influenza A & B, respiratory syncytial virus (RSV), influenza For 1, 2 and 3 and Adenovirus are often the most common. Symptoms of respiratory disease caused by Adenovirus includes from the common cold to pneumonia, "croup", and bronchitis. There are 47 serotype of adenovirus, causing different disease from conjunctivitis, bronchitis, pneumonia, diarrhea and other symptoms. Among them, the serotypes of 8, 14, 16,and 17 have been shown to cause conjunctivitis, while serotypes 7, 14, 21 cause respiratory symptoms. The antibodies used in the current test kit has a wide range of reactivity against many serotypes of adenovirus, including serotypes of 1, 2, 3, 4, 5, 6, 7, 8, 11, 14, 16, 17, 19 and 37.

The Adenovirus Antigen Rapid Test Cassette (Swab) is a rapid chromatographic immunoassay for the qualitative detection of adenovirus in eye conjunctive swab, throat swab and nasal swab specimen, providing results in 15 minutes. The test utilizes antibody specific for adenovirus to selectively detect adenovirus from eye conjunctive swab, throat swab and nasal swab specimens.

How to use?

Allow the test, specimen, extraction buffer to equilibrate to room temperature (15-30°C) prior to testing.

• Remove the test cassette from the sealed foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed immediately after opening the foil pouch.
• Place the Extraction Tube in the workstation. Hold the extraction reagent bottle upside down vertically. Squeeze the bottle and let the solution drop into the extraction tube freely without touching the edge of the tube. Add 10 drops of solution (Approx. 400μl) to the Extraction Tube. See illustration 1.
• Place the swab specimen in the Extraction Tube. Rotate the swab for approximately 10 seconds while pressing the head against the inside of the tube to release the antigen in the swab. See illustration 2.
• Remove the swab while squeezing the swab head against the inside of the Extraction Tube as you remove it to expel as much liquid as possible from the swab. Discard the swab in accordance with your biohazard waste disposal protocol. See illustration 3.
• Fit the dropper tip on top of the extraction tube. Place the test cassette on a clean and level surface. See illustration 4
• Add three drops of the solution (approx.120μl) to the sample well and then start the timer. Read results at 15 minutes and disregard after 60 minutes. A positive result may be visible at 3 minutes; however, the complete reaction time of 15 minutes is required to confirm a negative result.

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE: * Two distinct colored lines appear. One color line should be in the control region (C) and another color line should be in the test region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of adenovirus present in the specimen. Therefore, any shade of red in the test region should be considered positive.

NEGATIVE: One color line appears in the control region (C). No apparent red or pink line appears in the test region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.