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Infectious Disease Testing Rapid Detection of Vibrio Cholerae O1 O139 in Human Feces chromatographic immunoassay

Brand Name:	ALLTEST
MOQ:	N/A
Price:	negotiation
Payment Terms:	Ex works
Supply Ability:	2000mg/month

Detail Information

Product Description

Detail Information

Sample Type:

Feces

Test Result:

Accurate And Reliable

Test Equipment Operation:

Simple

Type:

Diagnostic Test

Packaging Details:

0.5mg/tube 1mg/tube, 2mg/tube、5mg/tube、10mg/tube、20mg/tube、30mg/tube

Supply Ability:

2000mg/month

Highlight:

recombinant antigen

Recombinant Proteins

Product Description

A rapid test for the qualitative detection of Vibrio Cholerae O1 Inaba, Vibrio Cholerae

O1 Ogawa, Vibrio Cholerae O139 in human feces.

For professional in vitro diagnostic use only.

【INTENDED USE】

The Vibrio Cholerae O139, O1 Ogawa & Inaba Combo Rapid Test (Feces) is a rapid

chromatographic immunoassay for the qualitative detection of Vibrio Cholerae O1

Inaba, Vibrio Cholerae O1 Ogawa and Vibrio Cholerae O139 antigen in human feces

to aid in the diagnosis of Vibrio Cholerae infection.

【SUMMARY】

Cholera is an acute watery diarrheal disease caused mainly by Vibrio Cholerae

serogroup O1 and less commonly by V. Cholerae O139. Cholerae can lead to severe

diarrhea and death if untreated. The etiological agent of Cholerae has been identified

as Vibrio Cholerae (V. Cholerae), a gram negative bacterium, which is generally

transmittd to humans via contaminated water and food. And Cholerae is thus

predominantly associated with lack of safe drinking water, proper sanitation and

personal hygiene. Cholerae is an important public health problem in many parts of

Asia, Africa and Latin America. 1, 2 Globally, 3–5 million cases and over 100,000 deaths

occur annually due to Cholerae. 3 According to the antigenic components of bacteria,

there are three serotypes, namely rice leaf type, Ogawa type and Yandao type.The

main difference between the epidemiology of Cholerae during past years was variation

between serotypes from Ogawa to Inaba. 4 The emergence of V. Cholerae O1 serotype

Inaba can caused outbreaks of Cholerae. 5 Countries facing complex emergencies are

more vulnerable to Cholerae outbreaks. 6 It is critical to determine as quickly as

possible the presence of V. Cholerae O1 Inaba in clinical specimens, water, and food

so that appropriate monitoring and effective preventive measures can be undertaken

by public health authorities.

The Vibrio Cholerae O139, O1 Ogawa & Inaba Combo Rapid Test (Feces) is a rapid

chromatographic immunoassay for the qualitative detection of Vibrio Cholerae O1

Inaba, Vibrio Cholerae O1 Ogawa, Vibrio Cholerae O139 in human feces, providing

results in 10 minutes. The test utilizes antibodies specific for V. Cholerae antigens to

selectively detect V. Cholerae antigens in human feces.

【PRINCIPLE】

The Combo Test comprises of three parts and details of each part are given herewith.

The Vibrio Cholerae O1 Inaba Test (Feces):

The Vibrio Cholerae O1 Inaba Rapid Test Cassette (Feces) is a qualitative, lateral flow

immunoassay for the detection of V. Cholerae Inaba antigens in human feces. In this

experiment, the membrane was pre-coated with anti-V. Cholerae Inaba antibody in the

test line area. During testing, the specimen reacts with the particle coated with anti-V.

Cholerae Inaba antibody. The mixture migrates upward on the membrane by capillary

action to react with anti-V. Cholerae Inaba antibody on the membrane and generate a

colored line. The presence of this colored line in the test region indicates a positive

result, while its absence indicates a negative result. To serve as a procedural control,

a colored line will always appear in the control line region indicating that proper volume

of specimen has been added and membrane wicking has occurred.

The Vibrio Cholerae O1 Ogawa Test (Feces):

The Vibrio Cholerae O1 Ogawa Rapid Test Cassette (Feces) is a qualitative, lateral

flow immunoassay for the detection of V. Cholerae Ogawa antigens in human feces. In

this experiment, the membrane was pre-coated with anti-V. Cholerae Ogawa antibody

in the test line area.During testing, the specimen reacts with the particle coated with

anti-V. Cholerae Ogawa antibody. The mixture migrates upward on the membrane by

capillary action to react with anti-V. Cholerae Ogawa antibody on the membrane and

generate a colored line. The presence of this colored line in the test region indicates a

positive result, while its absence indicates a negative result. To serve as a procedural

control, a colored line will always appear in the control line region indicating that proper

volume of specimen has been added and membrane wicking has occurred.

The Vibrio Cholerae O139 Test (Feces):

The Vibrio Cholerae O139 Rapid Test Cassette (Feces) is a qualitative, lateral flow

immunoassay for the detection of Vibrio Cholerae O139 antigens in human feces. In

this test, the membrane is pre-coated with anti-Vibrio Cholerae O139 antibody on the

test line region of the test. During testing, the specimen reacts with the particle coated

with anti-Vibrio Cholerae O139 antibody. The mixture migrates upward on the

membrane by capillary action to react with anti-Vibrio Cholerae O139 antibodies on

the membrane and generate a colored line. The presence of this colored line in the

test region indicates a positive result, while its absence indicates a negative result. To

serve as a procedural control, a colored line will always appear in the control line

region indicating that proper volume of specimen has been added and membrane

wicking has occurred.

【REAGENTS】

The Combo Test comprises of three parts and details of each part are given herewith

The Vibrio Cholerae O1 Inaba Rapid Test Cassette contains monoclonal anti-V.

Cholerae Inaba antibody coated particles and monoclonal anti-V. Cholerae Inaba

antibody coated on the membrane.

The Vibrio Cholerae O1 Ogawa Rapid Test Cassette contains monoclonal anti-V.

Cholerae Ogawa antibody coated particles and monoclonal anti-V. Cholerae Ogawa

antibody coated on the membrane.

The Vibrio Cholerae O139 Rapid Test Cassette contains monoclonal anti-Vibrio

Cholerae O139 antibody coated particles and monoclonal anti-Vibrio Cholerae O139

antibody coated on the membrane.

【PRECAUTIONS】

• For professional in vitro diagnostic use only. Do not use after the expiration date.

• The test should remain in the sealed pouch until use.

• Do not eat, drink or smoke in the area where the specimens or kits are handled.

• Handle all specimens as if they contain infectious agents. Observe established

precautions against microbiological hazards throughout all procedures and follow

the standard procedures for proper disposal of specimens.

• Wear protective clothing such as laboratory coats, disposable gloves and eye

protection when specimens are assayed.

• The used test should be discarded according to local regulations.

• Humidity and temperature may adversely affect results.

【STORAGE AND STABILITY】

The kit can be stored at room temperature or refrigerated (2-30°C). The test cassette

is stable through the expiration date printed on the sealed pouch. The test cassette

must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the

expiration date.

【SPECIMEN COLLECTION AND PREPARATION】

For feces sample:

• The feces specimen must be collected in clean, dry, waterproof container containing

no detergents, preservatives or transport media.

• Bring the necessary reagents to room temperature before use.

• If specimens are to be shipped, they should be packed in compliance with local

regulations covering the transportation of etiologic agents.

【MATERIALS】

Materials Provided

• Test cassettes

• Droppers

• Package insert

• Specimen collection tubes with extraction buffer

Materials Required But Not Provided

• Timer

• Pipette and disposable tips (optional)

• Centrifuge

• Specimen collection containers

【DIRECTIONS FOR USE】

Allow the test cassette, specimen, buffer and/or control to reach room

temperature (15-30°C) prior to testing.

1. To collect fecal specimens:

Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen

collection container to obtain maximum antigens (if present). Best results will be

obtained if the assay is performed within 6 hours after collection. Specimen

collected may be stored for 3 days at 2-8°C if not tested within 6 hours. For long

term storage, specimens should be kept below -20°C.

2. To process fecal specimens:

• For Solid Specimens:

Unscrew the cap of the specimen collection tube,then randomly stab the

specimen collection applicator into the fecal specimen in at least 3 different

sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not

scoop the fecal specimen.

• For Liquid Specimens:

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of

the liquid feces (approximately 80 μL) into the specimen collection tube

containing the extraction buffer.

Tighten the cap onto the specimen collection tube, and then shake the specimen

collection tube vigorously to mix the specimen and the extraction buffer. Leave

the tube alone for 2 minutes.

3. Bring the pouch to room temperature before opening it. Remove the test cassette

from the foil pouch and use it within one hour. Best results will be obtained if the test

is performed immediately after opening the foil pouch.

4. Hold the specimen collection tube upright and open the cap onto the specimen

collection tube. Invert the specimen collection tube and transfer 2 full drops of the

extracted specimen (approximately 80 μL) to each specimen well (S) of the test

cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S).

See illustration below.

5. Read results at 10 minutes after dispensing the specimen. Do not read results

after 20 minutes.

Note: If the specimen does not migrate (presence of particles), centrifuge the

extracted specimens contained in the extraction buffer vial. Collect the supernatant,

dispense 80 μL of supernatant into each specimen well (S) of a new test cassette

and start afresh following the instructions mentioned above.

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products details

Infectious Disease Testing Rapid Detection of Vibrio Cholerae O1 O139 in Human Feces chromatographic immunoassay

Infectious Disease Testing Rapid Detection of Vibrio Cholerae O1 O139 in Human Feces chromatographic immunoassay

recombinant antigen

Recombinant Proteins